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          The neigh starts out as a squeal, but ends up a nicker.  The neigh is not a sound of fear.  It is used when a horse is being separated from others

"IS ANYONE ELSE THERE?"~   The horse neighs with it's head high, looking around for other horses or people.  The horse usually neighs several times (if the horse neighs after a companion has answered it's neigh, it is usually saying "Where are you?".
"I AM HERE"~   A returned nicker made by a fellow horse who hears the original horse's question.  This response is also a nicker, meant to tell the other horse that it's not alone.